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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital role in advertising and marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two supplemental splice variants had been claimed but specific nucleotide sequence information is not nevertheless out there.
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Blocking peptides are peptides that bind specially on the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded by the antibody.
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This tutorial outlines further feasible optimization of cellular and immunoassay parameters to be sure the very best results are...
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This guideline outlines further more probable optimization of mobile and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind on the epitope over the target protein. By comparing the staining within the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Info Cyclin E belongs to the remarkably conserved cyclin loved ones, whose members are characterised by a dramatic periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinct expression and degradation designs which contribute towards the temporal coordination of each mitotic event. Cyclin E sorts a posh with and functions as being a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S read more transition. This protein accumulates for the G1-S section boundary and is particularly degraded as cells progress via S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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